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pcpgl basic vector  (New England Biolabs)


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    Structured Review

    New England Biolabs pcpgl basic vector
    Pcpgl Basic Vector, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcpgl-basic/pmc09380165-63-39-48
    Average 86 stars, based on 1 article reviews
    pcpgl basic vector - by Bioz Stars, 2026-10
    86/100 stars

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    Related Articles

    Amplification:

    Article Title: Epigenetic control of ataxin‐1 in multiple sclerosis
    Article Snippet: The Renilla luciferase reporter vector pRL‐TK was purchased from Promega. .. The four DMRs identified within the ATXN1 gene by BS‐seq were amplified from genomic DNA with Phusion High‐Fidelity DNA Polymerase (New England Biolabs) using the primers listed in Table , and the PCR products were then cloned into the pCpGL‐basic vector using the restriction enzymes SpeI and NcoI (New England Biolabs). ..

    Polymerase Chain Reaction:

    Article Title: Epigenetic control of ataxin‐1 in multiple sclerosis
    Article Snippet: The Renilla luciferase reporter vector pRL‐TK was purchased from Promega. .. The four DMRs identified within the ATXN1 gene by BS‐seq were amplified from genomic DNA with Phusion High‐Fidelity DNA Polymerase (New England Biolabs) using the primers listed in Table , and the PCR products were then cloned into the pCpGL‐basic vector using the restriction enzymes SpeI and NcoI (New England Biolabs). ..

    Article Title: Imprinting of Skin/Inflammation Homing in CD4+ T Cells Is Controlled by DNA Methylation within the Fucosyltransferase 7 Gene.
    Article Snippet: .. Cloning of luciferase constructs, transient transfection of primary T cells, and luciferase assay PCR-amplified constructs were cloned into the CG-free pCpGL basic plasmid (20) by restriction digest with SpeI and BglII (New England Biolabs, Frankfurt am Main, Germany). ..

    Clone Assay:

    Article Title: Epigenetic control of ataxin‐1 in multiple sclerosis
    Article Snippet: The Renilla luciferase reporter vector pRL‐TK was purchased from Promega. .. The four DMRs identified within the ATXN1 gene by BS‐seq were amplified from genomic DNA with Phusion High‐Fidelity DNA Polymerase (New England Biolabs) using the primers listed in Table , and the PCR products were then cloned into the pCpGL‐basic vector using the restriction enzymes SpeI and NcoI (New England Biolabs). ..

    Article Title: Imprinting of Skin/Inflammation Homing in CD4+ T Cells Is Controlled by DNA Methylation within the Fucosyltransferase 7 Gene.
    Article Snippet: .. Cloning of luciferase constructs, transient transfection of primary T cells, and luciferase assay PCR-amplified constructs were cloned into the CG-free pCpGL basic plasmid (20) by restriction digest with SpeI and BglII (New England Biolabs, Frankfurt am Main, Germany). ..

    Cloning:

    Article Title: Imprinting of Skin/Inflammation Homing in CD4+ T Cells Is Controlled by DNA Methylation within the Fucosyltransferase 7 Gene.
    Article Snippet: .. Cloning of luciferase constructs, transient transfection of primary T cells, and luciferase assay PCR-amplified constructs were cloned into the CG-free pCpGL basic plasmid (20) by restriction digest with SpeI and BglII (New England Biolabs, Frankfurt am Main, Germany). ..

    Luciferase:

    Article Title: Imprinting of Skin/Inflammation Homing in CD4+ T Cells Is Controlled by DNA Methylation within the Fucosyltransferase 7 Gene.
    Article Snippet: .. Cloning of luciferase constructs, transient transfection of primary T cells, and luciferase assay PCR-amplified constructs were cloned into the CG-free pCpGL basic plasmid (20) by restriction digest with SpeI and BglII (New England Biolabs, Frankfurt am Main, Germany). ..

    Construct:

    Article Title: Imprinting of Skin/Inflammation Homing in CD4+ T Cells Is Controlled by DNA Methylation within the Fucosyltransferase 7 Gene.
    Article Snippet: .. Cloning of luciferase constructs, transient transfection of primary T cells, and luciferase assay PCR-amplified constructs were cloned into the CG-free pCpGL basic plasmid (20) by restriction digest with SpeI and BglII (New England Biolabs, Frankfurt am Main, Germany). ..

    Transfection:

    Article Title: Imprinting of Skin/Inflammation Homing in CD4+ T Cells Is Controlled by DNA Methylation within the Fucosyltransferase 7 Gene.
    Article Snippet: .. Cloning of luciferase constructs, transient transfection of primary T cells, and luciferase assay PCR-amplified constructs were cloned into the CG-free pCpGL basic plasmid (20) by restriction digest with SpeI and BglII (New England Biolabs, Frankfurt am Main, Germany). ..

    Plasmid Preparation:

    Article Title: Imprinting of Skin/Inflammation Homing in CD4+ T Cells Is Controlled by DNA Methylation within the Fucosyltransferase 7 Gene.
    Article Snippet: .. Cloning of luciferase constructs, transient transfection of primary T cells, and luciferase assay PCR-amplified constructs were cloned into the CG-free pCpGL basic plasmid (20) by restriction digest with SpeI and BglII (New England Biolabs, Frankfurt am Main, Germany). ..

    Purification:

    Article Title: Effects of salinity stress on methylation of the liver genome and complement gene in large yellow croaker (Larimichthys crocea).
    Article Snippet: Salinity is an important environmental factor that affects the yield and quality of large yellow croaker (Larimichthys crocea) during aquaculture.. Here, whole-genome bisulfite sequencing (WGBS), RNA-seq, bisulfite sequencing PCR (BSP), quantitative real-time PCR (qPCR), and dual luciferase reporter gene detection technologies were used to analyze the DNA methylation characteristics and patterns of the liver genome, the expression and methylation levels of important immune genes in large yellow croaker in response to salinity stress.. The results of WGBS showed that the cytosine methylation of CG type was dominant, CpGIsland and repeat regions were important regions where DNA methylation occurred, and the DNA methylation in upstream 2k (2000bp upstream of the promoter) and repeat regions had different changes in the liver tissue of large yellow croaker in the response to the 12‰, 24‰, 36‰ salinity stress of 4 w (weeks).



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    New England Biolabs pcpgl basic reporter gene construct
    TET2 restores EBNA2 activation of the methylated LMP1 promoter. The ability of EBNA2 to activate the unmethylated, methylated, and 5hmC-modified forms of <t>the</t> <t>EBV</t> LMP1 promoter was analyzed using reporter gene assays. LMP1p was cloned upstream of the luciferase gene in the CpG-free vector <t>pCpGL-basic</t> and was methylated (dark gray) or mock treated (light gray) in vitro. The construct was transfected into EBV− BJAB cells with and without TET2, which converts 5mC into 5hmC (black), in the presence and absence of EBNA2. Luciferase assays were performed 2 days posttransfection. The fold luciferase activation is shown relative to the activity of the unmethylated vector transfected with control vectors, which is set to 1. Error bars indicate standard errors calculated from 2 replicate experiments consisting of triplicate conditions.
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    TET2 restores EBNA2 activation of the methylated LMP1 promoter. The ability of EBNA2 to activate the unmethylated, methylated, and 5hmC-modified forms of the EBV LMP1 promoter was analyzed using reporter gene assays. LMP1p was cloned upstream of the luciferase gene in the CpG-free vector pCpGL-basic and was methylated (dark gray) or mock treated (light gray) in vitro. The construct was transfected into EBV− BJAB cells with and without TET2, which converts 5mC into 5hmC (black), in the presence and absence of EBNA2. Luciferase assays were performed 2 days posttransfection. The fold luciferase activation is shown relative to the activity of the unmethylated vector transfected with control vectors, which is set to 1. Error bars indicate standard errors calculated from 2 replicate experiments consisting of triplicate conditions.

    Journal: Journal of Virology

    Article Title: Restricted TET2 Expression in Germinal Center Type B Cells Promotes Stringent Epstein-Barr Virus Latency

    doi: 10.1128/JVI.01987-16

    Figure Lengend Snippet: TET2 restores EBNA2 activation of the methylated LMP1 promoter. The ability of EBNA2 to activate the unmethylated, methylated, and 5hmC-modified forms of the EBV LMP1 promoter was analyzed using reporter gene assays. LMP1p was cloned upstream of the luciferase gene in the CpG-free vector pCpGL-basic and was methylated (dark gray) or mock treated (light gray) in vitro. The construct was transfected into EBV− BJAB cells with and without TET2, which converts 5mC into 5hmC (black), in the presence and absence of EBNA2. Luciferase assays were performed 2 days posttransfection. The fold luciferase activation is shown relative to the activity of the unmethylated vector transfected with control vectors, which is set to 1. Error bars indicate standard errors calculated from 2 replicate experiments consisting of triplicate conditions.

    Article Snippet: The EBV LMP1 ED-L1 promoter inserted into the pCpGL-basic reporter gene construct was methylated or mock treated in vitro using CpG methyltransferase M.SssI (New England BioLabs [NEB]) according the manufacturer's instructions.

    Techniques: Activation Assay, Methylation, Modification, Clone Assay, Luciferase, Plasmid Preparation, In Vitro, Construct, Transfection, Activity Assay