pcpgl basic vector (New England Biolabs)
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Pcpgl Basic Vector, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcpgl-basic/pmc09380165-63-39-48
Average 86 stars, based on 1 article reviews
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Amplification:Article Title: Epigenetic control of ataxin‐1 in multiple sclerosis Article Snippet: The Renilla luciferase reporter vector pRL‐TK was purchased from Promega. .. The four DMRs identified within the ATXN1 gene by BS‐seq were amplified from genomic DNA with Phusion High‐Fidelity DNA Polymerase (New England Biolabs) using the primers listed in Table , and the PCR products were then cloned into the Polymerase Chain Reaction:Article Title: Epigenetic control of ataxin‐1 in multiple sclerosis Article Snippet: The Renilla luciferase reporter vector pRL‐TK was purchased from Promega. .. The four DMRs identified within the ATXN1 gene by BS‐seq were amplified from genomic DNA with Phusion High‐Fidelity DNA Polymerase (New England Biolabs) using the primers listed in Table , and the PCR products were then cloned into the Article Title: Imprinting of Skin/Inflammation Homing in CD4+ T Cells Is Controlled by DNA Methylation within the Fucosyltransferase 7 Gene. Article Snippet: .. Cloning of luciferase constructs, transient transfection of primary T cells, and luciferase assay PCR-amplified constructs were cloned into the CG-free Clone Assay:Article Title: Epigenetic control of ataxin‐1 in multiple sclerosis Article Snippet: The Renilla luciferase reporter vector pRL‐TK was purchased from Promega. .. The four DMRs identified within the ATXN1 gene by BS‐seq were amplified from genomic DNA with Phusion High‐Fidelity DNA Polymerase (New England Biolabs) using the primers listed in Table , and the PCR products were then cloned into the Article Title: Imprinting of Skin/Inflammation Homing in CD4+ T Cells Is Controlled by DNA Methylation within the Fucosyltransferase 7 Gene. Article Snippet: .. Cloning of luciferase constructs, transient transfection of primary T cells, and luciferase assay PCR-amplified constructs were cloned into the CG-free Cloning:Article Title: Imprinting of Skin/Inflammation Homing in CD4+ T Cells Is Controlled by DNA Methylation within the Fucosyltransferase 7 Gene. Article Snippet: .. Cloning of luciferase constructs, transient transfection of primary T cells, and luciferase assay PCR-amplified constructs were cloned into the CG-free Luciferase:Article Title: Imprinting of Skin/Inflammation Homing in CD4+ T Cells Is Controlled by DNA Methylation within the Fucosyltransferase 7 Gene. Article Snippet: .. Cloning of luciferase constructs, transient transfection of primary T cells, and luciferase assay PCR-amplified constructs were cloned into the CG-free Construct:Article Title: Imprinting of Skin/Inflammation Homing in CD4+ T Cells Is Controlled by DNA Methylation within the Fucosyltransferase 7 Gene. Article Snippet: .. Cloning of luciferase constructs, transient transfection of primary T cells, and luciferase assay PCR-amplified constructs were cloned into the CG-free Transfection:Article Title: Imprinting of Skin/Inflammation Homing in CD4+ T Cells Is Controlled by DNA Methylation within the Fucosyltransferase 7 Gene. Article Snippet: .. Cloning of luciferase constructs, transient transfection of primary T cells, and luciferase assay PCR-amplified constructs were cloned into the CG-free Plasmid Preparation:Article Title: Imprinting of Skin/Inflammation Homing in CD4+ T Cells Is Controlled by DNA Methylation within the Fucosyltransferase 7 Gene. Article Snippet: .. Cloning of luciferase constructs, transient transfection of primary T cells, and luciferase assay PCR-amplified constructs were cloned into the CG-free Purification:Article Title: Effects of salinity stress on methylation of the liver genome and complement gene in large yellow croaker (Larimichthys crocea). Article Snippet: Salinity is an important environmental factor that affects the yield and quality of large yellow croaker (Larimichthys crocea) during aquaculture.. Here, whole-genome bisulfite sequencing (WGBS), RNA-seq, bisulfite sequencing PCR (BSP), quantitative real-time PCR (qPCR), and dual luciferase reporter gene detection technologies were used to analyze the DNA methylation characteristics and patterns of the liver genome, the expression and methylation levels of important immune genes in large yellow croaker in response to salinity stress.. The results of WGBS showed that the cytosine methylation of CG type was dominant, CpGIsland and repeat regions were important regions where DNA methylation occurred, and the DNA methylation in upstream 2k (2000bp upstream of the promoter) and repeat regions had different changes in the liver tissue of large yellow croaker in the response to the 12‰, 24‰, 36‰ salinity stress of 4 w (weeks). |
